No, but service (before main disconnect and OCPD) and non-service wires are not permitted in the same conduit or other raceway.
You are fine as far as the NEC is concerned. As GoldDigger points out, if these were service conductors it would be a different story.This is a conduit from a bus plug to a hot water generator. At the generator they increased the conduit size going to a disconnect switch and pulled both the line and load in it.